BANQUE INTERNATIONALE DE SUJETS DE THESE

Ecole Doctorale : BSB - Biologie, Santé, Biotechnologies

Identification of chromatin factors involved in transposable elements silencing
n°33

Encadrant de thèse :
Luisa DI STEFANO
Adresse mail :
luisa.di-stefano@univ-tlse3.fr
Laboratoire de rattachement :
MCD - Molecular, Cellular and Developmental Biology Unit
Adresse du site internet du laboratoire :
https://cbi-toulouse.fr/fr/
Directeur de laboratoire :
 Kerstin BYSTRICKY
Adresse mail :
 kerstin.bystricky@univ-tlse3.fr
École Doctorale :
 EDBSB
PAYS :

Chine
Colombie
Mexique - discipline :
Langue de la thèse :
anglais ,

Directeur de thèse :
Luisa DI STEFANO

Site Internet : http://cbi-toulouse.fr/fr/equipe-di-stefano
Descriptif : Transposable elements (TE) are mobile genetic elements able to hop from one genomic location to another. TEs constitute a sizeable portion of many eukaryotic genomes. Their tight regulation is necessary for normal development and their activation causes genomic instability and has been linked to ageing, cancer and neurodegenerative diseases. Chromatin is at the heart of TE regulation, however very little is known about the precise role of chromatin marks in TE silencing. Here, we propose to use the model organism Drosophila melanogaster to study the molecular mechanisms governing TE silencing. The student will combine proteomic approaches with genetic and genomic analysis to identify novel factors implicated in TE silencing and characterize their biological function and mechanisms of action at TE loci. Revealing the identity of the chromatin factors involved in TE regulation in epithelial tissues will improve our understanding of the roles of TE de-repression in aging and in pathology.

Publications relatives au sujet :
Lepesant JMJ, Iampietro C, Galeota E, Augé B, Aguirrenbengoa M, Mercé C, Chaubet C, Rocher V, Haenlin M, Waltzer L, Pelizzola M and Di Stefano L.. A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons. Nucleic Acids Research, 2020 Feb 20;48(3):1206-1224
Miles WO, Lepesant JMJ, Bourdeaux J, Texier M, Kerenyi MA, Nakakido M, Hamamoto R, Orkin SH, Dyson NJ and Di Stefano L. The LSD1 family of histone demethylases and the PUMILIO post-transcriptional repressor function in a complex regulatory feedback loop. Mol Cell Biol. 2015 Dec 15; 35(24):4199-211

Mots clés : Chromatin ;Transposable Elements ;Drosophila melanogaster ;Transcription ;Epithelia ;

Collaboration avec un laboratoire : MCD - Molecular, Cellular and Developmental Biology Unit
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